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GCSF Antibody / CSF3

CAT: 0800-V4471-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V4471-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09919
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the GCSF antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
CSF3/4594
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the GCSF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GCSF antibody is available for research use only.
Storage Conditions
Aliquot the GCSF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the GCSF antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human U-87 cell lysate with GCSF antibody (clone CSF3/4594) . Predicted molecular weight ~22 kDa.

UniProtKB · P09919

Granulocyte colony-stimulating factor

CSF3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09919
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSF3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
G-CSF
EC number
—
Processing
Precursor
Secondary accessions
A8MXR7
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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