Brain Creatine Kinase Antibody / Creatine kinase B / CKB

• Description:

  Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

• Specifications:

  ELISA (Order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P12277

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Hamster, Guinea pig

• Immunogen:

  Recombinant human full-length protein was used as the immunogen for the Brain Creatine Kinase antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CKBB/6568

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the Brain Creatine Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Brain Creatine Kinase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Brain Creatine Kinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Brain Creatine Kinase antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Image Legend:

  Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using Brain Creatine Kinase antibody (clone CKBB/6568) . Predicted molecular weight ~43 kDa.