CGA Antibody / CHGA / Chromogranin A

• Description:

  Chromogranin A (CGA) is an acidic protein which is reported to be widely expressed in neural tissues and in secretory granules of human endocrine cells e.g. parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas and C cells of the thyroid.GA is found in the large majority of epithelial NE tumors: pituitary adenomas, parathyroid and pancreatic islet cell tumors, medullary thyroid carcinoma, and carcinoid tumors.Together with synaptophysin, CGA is the most important marker for the identification of NE and neuronal tumors and NE/neuronal differentiation in other tumors.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P10645

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the CGA antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  CHGA/8929R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CGA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CGA antibody is available for research use only.

• Storage Conditions:

  Aliquot the CGA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CGA antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human pancreas tissue with CGA antibody (clone CHGA/8929R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.