Von Willebrand Factor Antibody

• Description:

  Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P04275

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human VWF protein was used as the immunogen for the von Willebrand Factor antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  VWF/4458

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the von Willebrand Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This von Willebrand Factor antibody is available for research use only.

• Storage Conditions:

  Aliquot the von Willebrand Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted, Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with vWF antibody (clone VWF/4458) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.