LAG3 Antibody / CD223

LAG3 Antibody / CD223

• Description: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved KIEELE motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.
• CAS Number: 9007-83-4
• UniProt: P18627
• Host: Mouse
• Immunogen: A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the Lymphocyte activation gene 3 antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: IHC-P
• Format: Purified
• Buffer: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Reconstitution: Aliquot the Lymphocyte activation gene 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This Lymphocyte activation gene 3 antibody is available for research use only.