Products for Research Use Only

NSE Antibody

CAT: 0800-V4223-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V4223-20UGSize:20 µg
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Description
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the NSE antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ENO2/7447
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NSE antibody is available for research use only.
Storage Conditions
Aliquot the NSE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the NSE antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using antibody to NSE gamma (ENO2/7447) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

UniProtKB · P09104

Gamma-enolase

ENOG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09104
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ENO2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
B7Z2X9, Q96J33
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmMembrane

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding
Product Datasheet
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