CD56 Antibody

• Description :

  This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt :

  P13591, P13592

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant partial protein (within amino acids 400-650) from the human protein was used as the immunogen for the CD56 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  NCAM/7524

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the CD56 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD56 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD56 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD56 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC staining of FFPE human cerebellum tissue with CD56 antibody (clone NCAM/7524) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.