Products for Research Use Only
RegisterLogin

P57Kip2 Antibody

CAT: 0800-V4155-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V4155-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P49918
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human p57Kip2 protein was used as the immunogen for the p57Kip2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
RKIP2/7238
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the p57Kip2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This p57Kip2 antibody is available for research use only.
Storage Conditions
Aliquot the p57Kip2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the p57Kip2 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
IHC staining of FFPE human bladder tissue with p57Kip2 antibody. Strong nuclear staining using rKIP2/7238 mAb at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Related Products

CatalogName
V5559-20UGP57 Antibody / p57Kip2
V5559-100UGP57 Antibody / p57Kip2
V5559SAF-100UGP57 Antibody / p57Kip2
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody
V8674SAF-100UGInhibin alpha Antibody / INHA antibody

Alternative Products

CatalogName
MBS8587366-01p57KIP2 Antibody
W0385-01p57kip2 (ABT214) Antibody
GTR18219212-01Rabbit p57kip2 Antibody
403308-01P57Kip2

Popular Products