MGMT Antibody

CAT: 0800-V4132SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V4132SAF-100UGSize:100 µg
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Description
Cancer chemotherapeutic alkylating agents (e.g. BCNU, ) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P16455
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.
Clonality
Monoclonal
Isotype
IgG κ
Clone
MGMT/8186R
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MGMT antibody is available for research use only.
Storage Conditions
Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the MGMT antibody should be determined by the researcher.
Location
Nucleus
Image Legend
IHC staining of FFPE human lymph node tissue with MGMT antibody (clone MGMT/8186R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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