MGMT Antibody

• Description :

  Cancer chemotherapeutic alkylating agents (e.g. BCNU, ) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

• UniProt :

  P16455

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG κ

• Clone :

  MGMT/8364R

• Applications :

  IHC-P, WB

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This MGMT antibody is available for research use only.

• Storage Conditions :

  Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the MGMT antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nucleus

• Image Legend :

  IHC staining of FFPE human prostate tissue with MGMT antibody (clone MGMT/8364R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.