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Vinculin Antibody

CAT: 0800-V4103-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V4103-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and Alpha-Actinin. In addition, vinculin, Talin and Alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P18206
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human Vinculin (VCL) protein was used as the immunogen for the Vinculin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RVCL/7287
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
Aliquot the Vinculin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Vinculin antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human lung tissue with Vinculin antibody (clone rVCL/7287) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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