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PILT Antibody / TJAP1

CAT: 0800-RQ7550Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7550Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tight junction-associated protein 1, also called 'Protein incorporated later into tight junctions' (PILT), is a protein that in humans is encoded by the TJAP1 gene. This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q5JTD0
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids K160-N557) was used as the immunogen for the PILT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PILT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PILT antibody is available for research use only.
Storage Conditions
After reconstitution, the PILT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PILT antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A549 cells with PILT antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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