DOCK1 Antibody

• Description :

  Dock180, (Dedicator of cytokinesis) also known as DOCK1, is a large (~180 kDa) protein involved in intracellular signalling networks. This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q14185

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  E. coli-derived recombinant human protein (amino acids S100-T1732) was used as the immunogen for the DOCK1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, FACS, IF, Direct ELISA

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the DOCK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This DOCK1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the DOCK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the DOCK1 antibody should be determined by the researcher.

• Location :

  Cytoplasmic

• Image Legend :

  Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with DOCK1 antibody (red) and DAPI nuclear stain (blue) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.