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CEP250 Antibody

CAT: 0800-RQ7157Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7157Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene. This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9BV73
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids Q563-K853) was used as the immunogen for the CEP250 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CEP250 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CEP250 antibody is available for research use only.
Storage Conditions
After reconstitution, the CEP250 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CEP250 antibody should be determined by the researcher.
Location
Cytoplasmic, perinuclear
Image Legend
IHC staining of FFPE human placental tissue with CEP250 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · Q9BV73

Centrosome-associated protein CEP250

CP250_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BV73
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CEP250
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
E1P5Q3, O14812, O60588, Q9H450
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCilium biogenesis/degradation

Cellular component

Cell projectionCiliumCytoplasmCytoskeleton

Domain

Coiled coil

Disease

Cone-rod dystrophyDeafnessDisease variant

PTM

Phosphoprotein

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