ALYREF Antibody / Tho Complex 4 / THOC4

• Description:

  Aly/REF export factor, also known as THO complex subunit 4, THOC4 and Tho4, is a protein that in humans is encoded by the ALYREF gene. The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

• UniProt:

  Q86V81

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids M1-S257) was used as the immunogen for the ALYREF antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS, IHC-P, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the ALYREF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This ALYREF antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the ALYREF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the ALYREF antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human A431 cells with ALYREF antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.