V5 tag, AlpHcAbs® Rabbit

• Description:

  Anti-V5 tag, AlpHcAbs® Rabbit antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Rabbit antibody reacts with the V5 tag sequence (GKPIPNPLLGLDST) selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production.

• Host:

  Alpaca

• Species Reactivity:

  Universal

• Immunogen:

  V5 tag fusion protein

• Conjugation:

  Non-conjugated

• Type:

  Alpaca monoclonal Antibody

• Applications:

  WB, ICC/IF, ELISA, IP, Flow Cyt

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:10,000-1:50,000 ;ELISA: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;IP: 1-2ug/sample ;Flow Cyt:1µg for 106 cells ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

• Purity:

  Recombinant Expression and Affinity purified

• Form:

  Liquid

• Buffer:

  10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

• Storage Conditions:

  Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C