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CCND1 Antibody

CAT: 0013-GTR18083262-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18083262-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.
UniProt
P24385
Reactivity
Human, Mouse
Immunogen
Recombinant full length human protein was used as the immunogen for this CCND1 antibody.
Clonality
Monoclonal
Clone
SPM587
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the CCND1 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

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