CD8a Antibody

CAT: 0013-GTR18080892-02Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18080892-02Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.

UniProt

P01732

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for this CD8a antibody.

Clonality

Monoclonal

Clone

C8/468

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P01732

T-cell surface glycoprotein CD8 alpha chain

CD8A_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P01732
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CD8A
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: B4DT80, D6W5M8, Q13970, Q4ZG17

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variant

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

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