Anti-Transglutaminase-3 (TG3) Antibody

Anti-Transglutaminase-3 (TG3) Antibody

• Description: Mouse Monoclonal Antibody specific to Transglutaminase-3 (TG3)
• Product Name Alternative: EC 2.3.2.13, Transglutaminase E, TG(E, TGE, TGase E, Transglutaminase-3, TGase-3 [Cleaved into: Protein-glutamine γ-glutamyltransferase E 50 kDa catalytic chain; Protein-glutamine γ-glutamyltransferase E 27 kDa non-catalytic chain]
• CAS Number: 9007-83-4
• Gene Name: TGM3
• NCBI Gene ID: TGM3
• UniProt: Q08188
• Cellular Locus: Cytoplasm
• Host: Mouse
• Reactivity: Human
• Immunogen: Recombinant human TG3 (accession no. Q08188).
• Target Antigen: Protein-glutamine γ-glutamyltransferase E
• Target: Transglutaminase-3 (TG3)
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, ICC, IHC
• Field of Research: Enzymes
• Purification Method: Purified by Protein A affinity chromatography
• Dilution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
• Format: Purified
• Form: Lyophilized
• Buffer: Tris Buffer
• Function: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. {ECO:0000250}.
• Additionnal Information: Immunoblotting: use at 1-5ug/mL. Bands of ~77kDa and ~30kDa are detected.
  
  Immunocytochemistry: use at 2-10ug/mL.
  
  These are recommended concentrations.
  
  Enduser should determine optimal concentrations for their applications.
• Storage Conditions: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
• Specificity: This antibody recognizes full-length human TG3 (77kDa) and the proteolysed form of human TG3 (30-47kDa).
• Formulation: Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
• Target Background: Transglutaminase-3 (TG3), an epidermal- type enzyme, is responsible for formation of the epidermis. During keratinocyte differentiation, TG1 and TG3 appear to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). These reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Furthermore, TG3 in hair follicles is involved in cross-linking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath. During differentiation in these processes, a zymogen form of TG3 (77 kDa) is activated by limited proteolysis with cathepsins S and/or L.