SARS-CoV-2 Spike RBD Protein, His Tag (XBB.1.5/Omicron) (MALS verified)

SARS-CoV-2 Spike RBD Protein, His Tag (XBB.1.5/Omicron) (MALS verified)

• Background: It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Description: SARS-CoV-2 Spike RBD Protein, His Tag (XBB.1.5/Omicron) (SPD-C5242) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: XBB.1.5).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Arg 319 - Lys 537
• Host: HEK 293
• Target: Spike RBD
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: SARS-CoV-2
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD Protein, His Tag (XBB.1.5/Omicron) (Cat. No. SPD-C5242) with a linear range of 8-63 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 26.6 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C