Human Laminin 221 Protein, premium grade

• Background:

  Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.

• Description:

  Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.

• Specifications:

  Human Laminin 221 Protein, premium grade, produced by co-expression of Laminin alpha2E8, Laminin beta2E8 and Laminin gamma1E8, has a calculated MW of 94.3 kDa, 51.3 kDa and 53.7 kDa. The non-reducing (NR) protein migrates as 118-133 kDa due to glycosylation.

• Host:

  HEK293

• Target:

  Laminin 221

• Conjugation:

  Unconjugated

• Source:

  Human

• Stability:

  -20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution; 2-8°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  0.01 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with a linear range of 0.2-20 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Additionnal Information:

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Host or Source:

  HEK293

• Species:

  Human

• Preservative:

  Trehalose