Anti-CIKS/Act1 (NT) Antibody

Anti-CIKS/Act1 (NT) Antibody

• Description: Rabbit Polyclonal Antibody specific to CIKS/Act1 (NT)
• Product Name Alternative: EC 2.3.2.27, Adapter protein CIKS, Connection to IKK and SAPK/JNK, E3 ubiquitin-protein ligase CIKS, Nuclear factor NF-kappa-B activator 1, ACT1, TRAF3-interacting protein 2
• CAS Number: 9007-83-4
• Gene Name: TRAF3IP2
• NCBI Gene ID: TRAF3IP2
• UniProt: O43734
• Host: Rabbit
• Reactivity: Human
• Immunogen: Peptide corresponding to aa 2-15 of human CIKS.
• Target Antigen: E3 ubiquitin ligase TRAF3IP2
• Target: CIKS/Act1 (NT)
• Clonality: Polyclonal
• Type: Antibody
• Applications: ELISA, WB, IHC-P, IF
• Field of Research: Neuroscience
• Purification Method: Purified by peptide immuno-affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed:19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed:19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828, PubMed:24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828). {PubMed:19825828, PubMed:24120361, PubMed:33723527}.
• Additionnal Information: Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 63 kD is detected.
  
  Positive control: Human placenta tissue lysate.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
• Specificity: This antibody recognizes an epitope at the N-terminus of human CIKS/Act1 (Connection to IKK and SAPK/JNK), an activator of IKK. CIKS/Act1 interacts directly with IKKg and also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). CIKS/Act1 is associated with inflammation and stress responses and is expressed in a variety of human tissues.
• Formulation: PBS, pH 7.4.
• Buffer pH: pH 7.4