D-Luciferin

D-Luciferin

• Product Name Alternative: D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin
• CAS Number: 2591-17-5
• UNSPSC Description: D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP[1]. We of er the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
• Target Antigen: Fluorescent Dye
• Type: Dye Reagents
• Related Pathways: Others
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/D-Luciferin.html
• Purity: 99.87
• Solubility: DMSO : 125 mg/mL (ultrasonic)|H2O : < 0.1 mg/mL|H2O : 9.09 mg/mL (ultrasonic;adjust pH to 9 with 1M NaOH)
• Smiles: O=C([C@@H]1N=C(C2=NC3=CC=C(O)C=C3S2)SC1)O
• Molecular Weight: 280.33
• References & Citations: [1]Giuseppe Meroni, et al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity. ARKIVOC 2009 (i) 265-288.|[2]Inoue Y, et al. Timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growthusing in vivo bioluminescence imaging. Int J Biomed Imaging. 2010;2010:471408.|[3]Rajesh Shinde, et al. Luciferin derivatives for enhanced in vitro and in vivo bioluminescence assays. Biochemistry. 2006 Sep 19;45(37):11103-12.Acta Pharm Sin B. 12 March 2022.|Autophagy. 2023 Sep 21.|Biomed Pharmacother. 2019 Sep;117:109126.|bioRxiv. 2023 Apr 10.|bioRxiv. 2024 August 16.|BMC Cancer. 2023 Oct 31;23(1):1047.|Cancer Lett. 2023 Sep 21;216403.|Cell Chem Biol. 2021 Nov 23;S2451-9456(21)00482-7.|Cell Death Dis. 2020 Sep 17;11(9):765.|Compos Part B-Eng. 2024 Feb 15, 271, 111162.|Hortic Res. 2022 Jan 20;uhab088.|Immun Inflamm Dis. 2024 Sep;12(9):e70005.|J Hazard Mater. 2024 Oct 31:480:136351.|New Phytol. 2022 Sep 2.|Parasit Vectors. 2023 Apr 24;16(1):139.|Plant Cell Environ. 2023 Mar 9.|Plant Commun. 2024 Apr 30:100937.|Plant Commun. 2024 Sep 2:101076.|Plant Physiol. 2021 Jun 11;186(2):1302-1317.|Plant Physiol. 2024 Sep 1:kiae464.|Research Square Print. December 19th, 2022.|SSRN. 2023 Sep 15.|Acta Pharm Sin B. 2023 Sep 1.|Acta Pharmacol Sin. 2021 Jul 29.|Asian J Pharm Sci. 10 June 2022.|Autophagy. 2022 Nov 30.|Cell Metab. 2022 Sep 7;S1550-4131(22)00359-X.|Gastro Hep Adv. 2022.|Int J Oncol. 2020 Mar;56(3):761-771.|J Nanobiotechnology. 2023 Oct 19;21(1):383.|J Transl Med. 2024 Aug 28;22(1):792.|Mil Med Res. 2023 Jul 25;10(1):34.|New Phytol. 2023 Jun 15.|Pharmacol Res. 2021 Apr;166:105527.|Plant Cell Environ. 2023 Feb 9.|Plant Physiol. 2023 Aug 11;kiad452.|Research Square Print. 2023 Feb 3.|SSRN. 2023 Mar 20.|Adv Funct Mater. 2023 Sep 15.|Biosensors (Basel). 2024 May 16;14(5):252.|Cell Mol Gastroenterol Hepatol. 2021;12(3):839-856.|J Leukoc Biol. 2019 Nov;106(5):1089-1100. |Plasma Process Polym. 2024 Oct 22.|Sci Adv. 2024 Aug 16;10(33):eado1533.|Sci China Life Sci. 2021 May 27;1-21.|Vet Res. 2021 Jun 26;52(1):95.
• Shipping Conditions: Room Temperature
• Storage Conditions: 4°C (Powder, protect from light)
• Clinical Information: No Development Reported