Recombinant Alpha-fucosidase / AlfC

• Description:

  Recombinant Alpha-fucosidase / AlfC_x000D_ Catalog number: B2017339_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 100 µg_x000D_ Molecular Weight or Concentration: 63.4 kDa_x000D_ Supplied as: Powder_x000D_ Applications: a molecular tool for various biochemical applications_x000D_ Storage: ˆ’80°C_x000D_ Keywords: α-fucosidase, AlfC, Alpha-L-fucosidase, Alpha-fucosidase_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Fan S, Li W, Zhang K, Zou X, Shi W, Liu Z, Tang C, Huang W, Tang F. Enhanced antibody-defucosylation capability of α-L-fucosidase by proximity-based protein fusion Biochem Biophys Res Commun. 2023 Feb 19;645:40-46._x000D_ 2: Klontz EH, Li C, Kihn K, Fields JK, Beckett D, Snyder GA, Wintrode PL, Deredge D, Wang LX, Sundberg EJ. Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation Nat Commun. 2020 Dec 4;11(1):6204._x000D_ 3: Curiel JA, Peirotén Á, Langa S, de Vega E, Blasco L, Landete JM. Characterization and stabilization of the α-L-fucosidase set from Lacticaseibacillus rhamnosus INIA P603 Appl Microbiol Biotechnol. 2022 Dec;106(24):8067-8077._x000D_ 4: Becerra JE, Rodríguez-Díaz J, Gozalbo-Rovira R, Palomino-Schätzlein M, Zúñiga M, Monedero V, Yebra MJ. Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine mBio. 2020 Jan 14;11(1):e02804-19._x000D_ 5: Prabhu SK, Li C, Zong G, Zhang R, Wang LX. Comparative studies on the substrate specificity and defucosylation activity of three α-l-fucosidases using synthetic fucosylated glycopeptides and glycoproteins as substrates Bioorg Med Chem. 2021 Jul 15;42:116243._x000D_ 6: Rodríguez-Díaz J, Rubio-del-Campo A, Yebra MJ. Lactobacillus casei ferments the N-Acetylglucosamine moiety of fucosyl-α-1,3-N-acetylglucosamine and excretes L-fucose Appl Environ Microbiol. 2012 Jul;78(13):4613-9._x000D_ 7: Curiel JA, de Vega E, Langa S, Peirotén Á, Landete JM. Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages Enzyme Microb Technol. 2024 Aug;178:110445._x000D_ 8: Rodríguez-Díaz J, Carbajo RJ, Pineda-Lucena A, Monedero V, Yebra MJ. Synthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei Appl Environ Microbiol. 2013 Jun;79(12):3847-50._x000D_ 9: Li C, Li T, Wang LX. Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases Methods Mol Biol. 2018;1827:367-380._x000D_ 10: Becerra JE, Coll-Marqués JM, Rodríguez-Díaz J, Monedero V, Yebra MJ. Preparative scale purification of fucosyl-N-acetylglucosamine disaccharides and their evaluation as potential prebiotics and antiadhesins Appl Microbiol Biotechnol. 2015 Sep;99(17):7165-76. _x000D_ _x000D_ Products Related to Recombinant Alpha-fucosidase / AlfC can be found at Proteins

• Short Description:

  Catalog Number: B2017339 (100 µg)_x000D_ Recombinant Alpha-fucosidase (AlfC) is an enzyme produced through recombinant DNA technology that catalyzes the hydrolysis of fucose-containing glycosidic bonds. It is produced by inserting the gene into an expression system for large-scale production. AlfC is used in biochemical research to study fucosylated glycoconjugates and in biotechnology for modifying glycoproteins and other glycoconjugates. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height :

  0.5

• CAS Number:

  9000-83-3