Recombinant Mouse Lactase-like protein (Lctl) , partial

Recombinant Mouse Lactase-like protein (Lctl) , partial

• CAS Number: 9000-83-3
• Gene Name: Lctl
• UniProt: Q8K1F9
• Expression Region: 21-540aa
• Organism: Mus musculus
• Target Sequence: AWRGPPEEASFYYGTFPPGFSWGVGSSAYQTEGAWDEDGKGPSIWDAFTHGRKEQVLGGDTADTACDSYYKVQEDIALLKELQVSHYRFSLSWPRLLPTGVRAEQVNKRGIKFYSDFIDALLKSNITPVVTLHHWDLPQMLQVAYGGWQNVSMTRYFSDYADLCFEVFGDRVKHWLTFSDPRTMVEKGYETGLHAPGLRLQGTGLYVAAHHIIKAHAQAWHSYNNTWRSKQHGLVGISLNCDWGEPVDIDNPDDIEAAERYLQFCLGWFANPIYAGDYPQVMKDHIGTKSAEQGLEMSRLPTFSLQEKSYLKGTSDFLGLGHFTTRYITQRKYPSHQGPSYQNDRDLVELVDPNWPEMGSPWLYSVPWGFRRLLNFAQTQYGDPPIYVTESGAPQKLHCTQFCDEWRIQYLKGYINEMLKAIKDGVDIKGYTSWSLLDKFEWEKGYADKYGFYYVEFNVRNKPRYPKASVQYYKEIITASGFPNPQEVESWRLKALETCSINNQMLATEPLLRHMHVASE
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: In Stock Protein
• Relevance: Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 64.8 kDa
• References & Citations: "Large-scale gene trapping in C57BL/6N mouse embryonic stem cells." Hansen G.M., Markesich D.C., Burnett M.B., Zhu Q., Dionne K.M., Richter L.J., Finnell R.H., Sands A.T., Zambrowicz B.P., Abuin A. Genome Res 18:1670-1679 (2008)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.