Goat anti Rabbit IgG IgA IgM (heavy and light chains)

• Background:

  The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin em (classes and both light chain types) including antibodies to common determinants, to s and to the surface determinants of the common Fab portion, as tested by immunoelectroimmunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

• Host:

  Goat

• Clone:

  Polyclonal

• Source:

  Purified normal IgG and fractions containing IgA, and IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

• Assay Principle:

  Precipitation assays. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.

• Form:

  Delipidated, heat inactivated lyophilized stable whole serum; No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal goat serum; No foreign proteins added; It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Storage Conditions:

  The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the product

• CAS Number:

  9007-83-4