Uncoated Human TNF-β (Tumor Necrosis Factor Beta) ELISA Kit

• Abbreviation:

  TNF-β

• UniProt:

  P01374

• Cross Reactivity:

  This kit recognizes TNF-β in samples.No significant cross-reactivity or interference between TNF-β and analogues was observed.

• Target:

  LT; LTA; TNFB; TNFSF1; TNF Superfamily; Member 1; Lymphotoxin Alpha

• Type:

  Sandwich-ELISA

• Applications:

  ELISA

• Field of Research:

  Cell Biology; Immunology; Neuroscience

• Assay Principle:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNF-β . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNF-β , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNF-β . You can calculate the concentration of TNF-β in the samples by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  24 h

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.63-1000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%

• Recovery:

  80%-120%

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  -20°C,12 months

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human TNF-β concentrations in serum, plasma and other biological fluids.