Hakai (AP)

• Reactivity:

  Canine

• Storage Conditions:

  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

• Specificity:

  Recognizes canine Hakai. On Western blots, it identifies a single band at ~70kD, as well as a band at ~75-80kD that likely represents the ubiquitinated form of the Hakai protein. Reactivity has been confirmed with MDCK cell lysates. Species sequence homology: Mouse and human.

• Short Name:

  [Hakai]