Recombinant Rat PRL Protein (His Tag)

• Background:

  Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.

• Abbreviation:

  PRL

• Synonyms:

  Lactotrope; LTH; Luteotropic Hormone; Prolactin precursor; PRL; Prolactin

• UniProt:

  P01237

• Accession Number:

  P01237

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Leu30-Cys266

• Field of Research:

  Metabolism; Developmental Biology; Neuroscience

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  26 kDa

• Observed Molecular Weight:

  28 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Rat