Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

• Synonyms: Cytochrome C Oxidase
• Field of Research: Mitochondria Function
• Detection Method: Colorimetric method
• Assay Principle: Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
• Assay Performance Time: 35 min
• Detection Range: 0.79-47.0 U/L
• Sensitivity: 0.79 U/L
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at -20°C for 12 months with shading light.