NrCAM Antibody (APC)

Mouse monoclonal to NrCAM (APC) . Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31. 1-q31. 2.

Neuronal cell adhesion molecule, Bravo, hBravo, KIAA0343, MGC138845, MGC138846, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM

Q810U4

Human, Mouse, Rat

Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical) . Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.

NrCAM

N364/51 (Formerly sold as S364-51)

APC

Cell Biology, Disease Biomarkers, Neuroscience

Protein G Purified

1 mg/ml

WB (1:1000), ICC/IF (1:100)

160kDa

Conjugated antibodies should be stored according to the product label

For research use only.

1 μg/ml was sufficient for detection of NrCAM in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

ICC, IF, WB

NP_001139503.1

Mouse

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

IgG2a