Biotinyl Tyramide

• Background:

  Biotinyl Tyramide is a biotin derivative that can be used as a reagent to amplify both IHC signals and in situ hybridization protocols (1). The secondary should be an HRP-labelled antibody or streptavidin conjugate; catalysis by the HRP results in the deposition of multiple tyramide molecules which can then be detected with a labeled streptavidin conjugate. Sensitivity has found to be 100 fold. It can be used with both chromogenic and fluorescent visualization methods (2).

• Description:

  Signal Amplifier

• Product Name Alternative:

  (3aS,4S,6aR) -Hexahydro-N-[2- (4-hydroxyphenyl) ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide; Biotin-phenol

• UNSPSC:

  41116105

• Type:

  Amplifier

• Source:

  Synthetic

• Field of Research:

  Cell Signaling | Neuroscience

• Purity:

  >98% (TLC) ; NMR (conforms)

• Weight:

  0.005

• Format:

  White powder

• Solubility:

  May be dissolved in DMSO (35 mg/ml); or ethanol (20 mg/ml)

• Molecular Formula:

  C18H25N3O3S

• Molecular Weight:

  363.5

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Bobrow M.N. et al., (1989) J. Immunol. Methods. 125:279. 2. Kim S.H., et al. (2003) J Histochem Cytochem. 51(1): 129-132.

• CAS Number:

  41994-02-9