Tau-441 (2N4R) P301S Mutant Filaments

Tau-441 (2N4R) P301S Mutant Filaments

• Background: Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
• Description: Human Recombinant Tau-441 (2N4R) P301S Mutant Protein Filaments
• Product Name Alternative: Tau filament, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Tau Protein Aggregate, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit
• UNSPSC: 12352202
• Gene ID: 4137
• Swiss Prot: P10636
• Accession Number: NP_005901.2
• Cellular Locus: Cytoplasm | Axolemma | Axolemma Plasma Membrane | Axon | Cell Body | Cell membrane | Cytoplasmic Ribonucleoprotein Granule | Cytoplasmic Side | Cytoskeleton | Cytosol | Dendrite | Growth cone | Microtubule | Microtubule Associated Complex | Neurofibrillary Tangle | Neuronal Cell Body | Nuclear Periphery | Nuclear Speck | Nucleus | Peripheral membrane protein | Plasma Membrane | Tubulin Complex
• Host: E. coli
• Origin Species: Human
• Target: Tau-441 (2N4R) P301S Mutant
• Conjugation: No tag
• Applications: WB, SDS-PAGE, In vivo assay, In vitro assay
• Purification Method: Ion-exchange Purified
• Concentration: 2 mg/ml
• Purity: >95%
• Weight: 0.2
• Length: Full length
• Buffer: PBS pH 7.4, 5 mM DTT, 2 mM MgCl2
• Molecular Weight: 45.84 kDa
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
• Additionnal Information: For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or the product datasheet for further information. Monomer source is catalog# SPR-327.
• References & Citations: 1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497. 4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18. 5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723. 6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.