DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody) : APC

CAT: 0400-SPC-720D-APCSize: 100 µgDry Ice: NoHazardous: No
CAT#:0400-SPC-720D-APCSize:100 µg
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Background
The DYKDDDDK tag, also known as the FLAG® tag, is a widely used epitope tag in molecular biology and protein research. Comprising eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), this hydrophilic sequence is engineered into recombinant proteins to facilitate detection, purification, and localization studies. Its small size minimizes interference with protein function, making it ideal for fusion protein applications in both prokaryotic and eukaryotic systems. Anti-DYKDDDDK tag antibodies, such as those equivalent to Sigma’s Anti-FLAG® M2, are monoclonal or polyclonal antibodies specifically designed to recognize the DYKDDDDK epitope with high affinity and specificity. These antibodies are essential tools in Western blotting, immunoprecipitation, immunofluorescence, and ELISA, enabling researchers to track tagged proteins with precision. The FLAG tag system is particularly valued for its compatibility with gentle elution conditions, preserving protein structure and function during purification. Additionally, the tag’s unique sequence ensures minimal cross-reactivity with endogenous proteins, enhancing signal clarity in complex biological samples. Anti-DYKDDDDK antibodies are validated across multiple species and applications, making them a cornerstone in proteomics, structural biology, and therapeutic protein development. Their robust performance and commercial availability have established them as a gold standard in epitope tagging technologies.
Description
Rabbit Anti- DYKDDDDK Tag Polyclonal
CAS Number
9007-83-4
Specifications
Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
Product Name Alternative
DDDDK epitope tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Host
Rabbit
Species Reactivity
Species Independent
Immunogen
Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH) .
Target
DYKDDDDK Tag
Clonality
Polyclonal
Conjugation
APC
Type
Polyclonal
Applications
WB
Validated Applications
IHC, ICC/IF
Field of Research
Tags and Cell Markers | Epitope Tags
Purification
Peptide Affinity Purified
Detection Range
A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000) ; optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
Conjugated antibodies should be stored according to the product label
Specificity
Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
Species
Species Independent

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MBS8000402-01DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): Biotin
MBS8000404-01DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): HRP
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MBS8000393-01DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): ATTO 488
MBS8000400-01DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-DYKDDDDK M2 Antibody): Alkaline Phosphatase
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