ENaC beta Antibody: PerCP

ENaC beta Antibody: PerCP

• Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
• Description: Rabbit Anti-Rat ENaC beta Polyclonal
• Specifications: Detects ~87kDa.
• Product Name Alternative: Neuroscience, Neurotransmission, Ion Channels, Sodium Channels, Epithelial Sodium Channels (ENaC)
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 24767
• Swiss Prot: P37090
• Accession Number: NP_036780
• Cellular Locus: Apical Cell Membrane
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Xenopus, Hamster
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Target: ENaC
• Clonality: Polyclonal
• Conjugation: PerCP
• Validated Applications: WB, IHC, ICC/IF, ELISA
• Purification: Protein A Purified
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.