Methylated Lysine Antibody

• Background:

  Methylated lysine is a key post-translational modification that plays a pivotal role in regulating gene expression, chromatin structure, and protein-protein interactions in the nervous system. Lysine residues on histone proteins can undergo mono-, di-, or tri-methylation, influencing transcriptional activation or repression depending on the site and degree of methylation. This epigenetic regulation is essential for neuronal development, synaptic plasticity, and memory formation. In neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease, aberrant lysine methylation patterns have been linked to dysregulated gene expression and impaired neuronal function. Enzymes that catalyze lysine methylation (lysine methyltransferases) and demethylation (lysine demethylases) are increasingly recognized as contributors to disease pathogenesis. Altered activity of these enzymes can disrupt chromatin dynamics, leading to neuronal vulnerability and degeneration. Targeting lysine methylation pathways offers a promising therapeutic strategy for modulating gene expression and restoring neuronal homeostasis. As research advances, methylated lysine serves as both a biomarker and a mechanistic link between epigenetic regulation and neurodegenerative progression.

• Description:

  Rabbit Anti-Methylated Lysine Polyclonal

• Specifications:

  Detects proteins containing methylated lysine residues.

• Product Name Alternative:

  Methylated Lysine, Methyl lysine, Dimethylysine, Trimethyl lysine, N epsilon dimethyl lysine

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Host:

  Rabbit

• Species Reactivity:

  Species Independent

• Immunogen:

  Methylated KLH Conjugated

• Target:

  Methylated Lysine

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | ICC/IF | IP | ELISA

• Validated Applications:

  WB, ICC/IF, IP, ELISA

• Field of Research:

  Cell Signaling | Post-translational Modifications | Methylation

• Purification:

  Protein A Purified

• Detection Range:

  0.2-0.5 µg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:20000), ICC/IF (1:200) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.4

• Buffer:

  PBS, 50% glycerol *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1 (3) : 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects proteins containing methylated lysine residues.

• Species:

  Species Independent

• CAS Number:

  9007-83-4