Recombinant Porcine reproductive and respiratory syndrome virus Nucleoprotein (N)

Recombinant Porcine reproductive and respiratory syndrome virus Nucleoprotein (N)

• CAS Number: 9000-83-3
• Gene Name: N
• UniProt: Q04558
• Expression Region: 1-128aa
• Organism: Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV)
• Target Sequence: MAGKNQSQKKKKSTAPMGNGQPVNQLCQLLGAMIKSQRQQPRGGQAKKKKPEKPHFPLAAEDDIRHHLTQTERSLCLQSIQTAFNQGAGTASLSSSGKVSFQVEFMLPVAHTVRLIRVTSTSASQGAS
• Tag: C-terminal 6xHis-tagged
• Source: E.coli
• Field of Research: Microbiology
• Assay Type: Developed Protein
• Relevance: Multifunctional protein which associates with itself through both covalent and non-covalent interactions to provide the basis for viral assembly. May play a role in the regulation of cellular gene expression during infection by interacting with host HIC. Plays a role in the inhibition of the dsRNA-mediated IFN induction pathway by inhibiting host IRF3 phosphorylation and nuclear translocation.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 14.8 kDa
• References & Citations: "Poly (A)-binding protein interacts with the nucleocapsid protein of porcine reproductive and respiratory syndrome virus and participates in viral replication." Wang X., Bai J., Zhang L., Wang X., Li Y., Jiang P. Antiviral Res. 96:315-323 (2012)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.