OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

• Description:

  The OxiSelect™ ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

• UNSPSC:

  41116100

• Detection Method:

  Fluorometric

• Features & Benefits:

  Quick ~1 hour protocol; Highly sensitive to 10 pM; Detects the presence of various ROS species

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  Upon receipt, store the DCFH-DA, DCF and Hydrogen Peroxide at -20°C. Avoid multiple freeze/thaw cycles. Store the Cell Lysis Buffer at 4°C.