Alpha Synuclein Antibody, Clone 3F8

CAT: 0400-SMC-532DSize: 100 µgDry Ice: NoHazardous: No
CAT#:0400-SMC-532DSize:100 µg
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Background
Alpha-synuclein (SNCA) is a neuronal protein predominantly expressed in the brain, where it is highly concentrated at presynaptic nerve terminals, suggesting a key role in synaptic transmission and plasticity (1) . In addition to its synaptic localization, alpha-synuclein is abundantly expressed in mitochondria-rich brain regions, including the olfactory bulb, hippocampus, striatum, and thalamus, implicating it in mitochondrial function and energy metabolism (2) . Functionally, alpha-synuclein has been shown to interact significantly with tubulin (3), indicating a potential role as a microtubule-associated protein involved in cytoskeletal organization and intracellular transport. This interaction may be critical for maintaining neuronal structure and function, particularly in long-range axonal projections. Importantly, alpha-synuclein is essential for normal cognitive development. Genetic or functional inactivation of SNCA has been associated with impairments in spatial learning and working memory, underscoring its role in higher-order brain functions (4) . Given its physiological importance and pathological aggregation in disorders such as Parkinson’s disease and dementia with Lewy bodies, alpha-synuclein remains a focal point in neurodegenerative disease research. Understanding its normal function and mechanisms of misfolding is vital for developing targeted diagnostics and therapeutics.
Description
Mouse Anti-Mouse Alpha Synuclein Monoclonal IgG1
CAS Number
9007-83-4
Specifications
Binds within the 101-140 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Does not detect truncated alpha Synuclein (aa 1-114)
Product Name Alternative
Alpha Synuclein, α-Synuclein, SNCA, Snca, SYN, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease familial 1, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4
Synonyms
Alpha Synuclein Antibody, Clone 3F8
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
20617
Swiss Prot
O55042
Accession Number
NP_001035916.1
Cellular Locus
Cytoplasm | Cytosol | Membrane | Nucleus | Cell Junction | Synapse
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Mouse alpha synuclein aggregate
Target
Alpha Synuclein
Clonality
Monoclonal
Isotype
IgG1
Clone
3F8
Conjugation
Unconjugated
Tissue Specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Type
Monoclonal
Applications
WB | DB | IHC | ICC/IF | ELISA
Validated Applications
WB, DB, IHC, ICC/IF, ELISA
Field of Research
Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy
Purification
Protein G Purified
Limit Of Detection
A 1:1000 dilution of SMC-532 was sufficient for detection of Alpha Synuclein in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000) ; DB (1:1000) ; IHC (1:200) ; ICC/IF (1:100) ; ELISA (1:1000) ; optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Binds within the 101-140 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Does not detect truncated alpha Synuclein (aa 1-114)
Background Reference 01
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013) . 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277 (3) : 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231 (1) : 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388 (6645) : 839-840. 6. Mezey E., et al. (1998) Nat Med. 4 (7) : 755-757.
Species
Human | Mouse | Rat
Location
Cytoplasm | Cytosol | Membrane | Nucleus | Cell Junction | Synapse
Isotype
IgG1
Immunogen Species
Mouse

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