Hexanoyl-Lysine adduct Antibody, Clone 5D9

• Background:

  Hexanoyl-lysine adduct (HEL) is a lipid peroxidation marker formed by the reaction of oxidized fatty acids with lysine residues on proteins. It serves as an early indicator of oxidative stress, a central feature in the pathogenesis of neurodegenerative diseases. In the brain, HEL accumulation reflects damage to neuronal membranes and proteins caused by reactive oxygen species (ROS) . Elevated levels of HEL have been detected in models of Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative conditions, correlating with disease progression and cognitive decline. HEL is particularly valuable as a biomarker due to its stability and specificity for lipid peroxidation. Its presence indicates oxidative damage at an early stage, often preceding overt neurodegeneration. This makes HEL a promising candidate for early diagnosis, disease monitoring, and evaluating the efficacy of antioxidant therapies. By highlighting the oxidative component of neurodegeneration, HEL contributes to a deeper understanding of disease mechanisms and supports the development of targeted interventions aimed at reducing oxidative injury in the nervous system.

• Description:

  Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1

• Specifications:

  Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

• Product Name Alternative:

  Hexanoyl-Lysine adduct, HEL (Hexanoyl-Lysine adduct), HEL, HEL Adduct, Hexanoyl-Lys adduct, Hexanoyl-Lys, Hexanoyl-Lysine (HEL) adduct, Hexanoyl-Lys (HEL), Hexanoyl Lysine adduct, Hexanoyl-Lysine adduct-modified protein, Hexanoyl-Lysine Adduct (HEL)

• Synonyms:

  Hexanoyl-Lysine adduct Antibody, Clone 5D9

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Host:

  Mouse

• Species Reactivity:

  Species Independent

• Immunogen:

  Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) .

• Target:

  Hexanoyl-Lysine adduct

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  5D9

• Conjugation:

  Unconjugated

• Type:

  Monoclonal

• Applications:

  WB | ICC/IF | FACS | FCM | ELISA | IHC

• Validated Applications:

  WB, ICC/IF, FACS, FCM, ELISA, IHC

• Field of Research:

  Cancer | Oxidative Stress | Lipid peroxidation

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-508 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000) ; FACS (1:50) ; FCM (1:50) ; IHC (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

• Background Reference 01:

  1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.

• Species:

  Species Independent

• CAS Number:

  9007-83-4

• Isotype:

  IgG1