TCP1 alpha Antibody: ATTO 488

TCP1 alpha Antibody: ATTO 488

• Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
• Description: Rat Anti-Mouse TCP1 alpha Monoclonal IgG2a
• Specifications: Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
• Product Name Alternative: p63 Antibody, Tcp-1 Antibody, TCP1 alpha Antibody, Ccta Antibody, TRic Antibody, Tp63 Antibody, 21454 Antibody, c-cpn Antibody, CCT Antibody, Tcp1 Antibody, ccpn Antibody, AI528772 Antibody, Cct1 Antibody, Tcp 1 Antibody, c cpn Antibody
• Synonyms: TCP1 alpha Antibody, Clone 91a: ATTO 488
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 21454
• Swiss Prot: P11983
• Accession Number: NP_038714.2
• Cellular Locus: Cytoplasm
• Host: Rat
• Species Reactivity: Human, Mouse, Rat, Bovine, Rabbit, Guinea Pig, Hamster, Dog, Pig, Yeast, C. elegans, Drosophila, Monkey
• Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
• Target: TCP1 alpha
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 91a
• Conjugation: ATTO 488
• Validated Applications: IHC, ICC/IF
• Purification: Protein G Purified
• Limit Of Detection: 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.