LRRK2 Antibody, Clone N231B/34: APC

• Background:

  LRRK2 (Leucine-Rich Repeat Kinase 2) is a large multidomain protein with kinase and GTPase activity, implicated in the pathogenesis of Parkinson’s disease (PD) and other neurodegenerative disorders. Mutations in LRRK2, such as G2019S, are among the most common genetic causes of familial and sporadic PD. LRRK2 regulates vesicle trafficking, autophagy, cytoskeletal dynamics, and immune responses. Its dysregulation leads to mitochondrial dysfunction, impaired lysosomal degradation, and neuroinflammation. LRRK2 is expressed in neurons and glial cells, and its activity is modulated by cellular stress and inflammatory signals. As a central node in neurodegenerative signaling networks, LRRK2 is a high-priority target for disease-modifying therapies in Parkinson’s and related disorders.

• Description:

  Mouse Anti-Human LRRK2 (Dardarin) Monoclonal IgG2a

• Specifications:

  Detects >200kDa.

• Product Name Alternative:

  LRRK2, Leucine-rich repeat kinase 2, Leucine-rich repeat serine/threonine-protein kinase 2, RIP7, RIPK7, PARK8, Dardarin, ROCO 2, Augmented in rheumatoid arthritis 17

• Synonyms:

  LRRK2/Dardarin Antibody, Clone N231B/34: APC

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  120892

• Swiss Prot:

  Q5S007

• Accession Number:

  NP_940980

• Cellular Locus:

  Cytoplasm | Membrane | Mitochondrion | Golgi Apparatus | Cell Projection | Axon | Dendrite | Endoplasmic Reticulum | Cytoplasmic Vesicle | Endosome | Lysosome

• Host:

  Mouse

• Species Reactivity:

  Human, Rat

• Immunogen:

  Fusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat. <30% identity with LRRK1.

• Target:

  LRRK2

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  N231B/34 (Formerly sold as S231B-34)

• Conjugation:

  APC

• Type:

  Monoclonal

• Applications:

  WB | ICC/IF | IHC

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Neuroscience | Neurodegeneration | Parkinson's Disease | Cell Signaling | Post-translational Modifications | Phosphorylation | Neuroscience | Neurodegeneration | Multiple System Atrophy

• Purification:

  Protein G Purified

• Limit Of Detection:

  10 µg/ml of SMC-445 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  95.46mM Phosphate, 2.48mM MES and 2mM EDTA

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects >200kDa.

• Species:

  Human | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Membrane | Mitochondrion | Golgi Apparatus | Cell Projection | Axon | Dendrite | Endoplasmic Reticulum | Cytoplasmic Vesicle | Endosome | Lysosome

• Isotype:

  IgG2a

• Immunogen Species:

  Human