GIT1 Antibody, Clone N39B/8

GIT1 (G-protein-coupled receptor kinase-interacting protein 1) is a highly conserved scaffold protein that plays a central role in coordinating intracellular signaling, cytoskeletal remodeling, and cell migration. It contains multiple interaction domains that enable binding to small GTPases (such as ARF, Rac, and Cdc42), kinases (including PAK and MEK), the Rho-family GEF PIX, and the focal adhesion protein paxillin. Through these interactions, GIT1 regulates focal adhesion dynamics and membrane protrusion formation. In the nervous system, GIT1 is enriched at synapses and is essential for synapse formation, dendritic spine morphogenesis, and synaptic plasticity. Its localization to cytoplasmic complexes and membrane structures supports its role in neuronal signaling and structural remodeling. Importantly, GIT1 has been implicated in the pathogenesis of Huntington’s disease (HD), a neurodegenerative disorder caused by mutations in the huntingtin (HTT) gene. GIT1 directly interacts with mutant HTT, enhancing its proteolysis and potentially contributing to the accumulation of toxic protein fragments. This interaction suggests that altered GIT1 distribution or function may exacerbate neuronal dysfunction and degeneration in HD. Given its dual role in cytoskeletal regulation and synaptic signaling, GIT1 is emerging as a critical molecular player in neurodegenerative disease research. Understanding its mechanisms may reveal novel therapeutic targets for modulating synaptic integrity and mitigating disease progression.

Mouse Anti-Rat GIT1 Monoclonal IgG1

9007-83-4

Detects ~90kDa. Does not cross-react with GIT2.

ARF GAP GIT1, ARF GTPase-activating protein, ARF GTPase-activating protein GIT1, CAT1, Cool-associated and tyrosine-phosphorylated protein 1, G protein-coupled receptor kinase-interactor 1, GIT 1, GIT1, GRK-interacting protein 1

GIT1 Antibody, Clone N39B/8

12352203

Non-hazardous

Non-hazardous

83709

Q9Z272.1

NP_114002.1

Cytoplasm

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1

GIT1

N39B/8 (Formerly sold as S39B-8)

Unconjugated

Monoclonal

WB | ICC/IF | IP

Neuroscience | Cell Markers | Neuron Markers | Cytoplasmic Markers | Cell Signaling | Scaffold Proteins | Post-translational Modifications | Phosphorylation

Protein G Purified

1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

1 mg/ml

WB (1:1000) ; optimal dilutions for assays should be determined by the user.

0.1

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475. 2. Manabe R., et al. (2002) J. Cell Sci. 115: 1497-1510. 3. Zhang H., et al. (2003) J. Cell Biol. 161: 131-142. 4. Goehler H., et al. (2004) Mol. Cell 15: 853-865. 5. Mende-Mueller L.M., et al. (2001) J. Neurosci. 21: 1830-1837.

Blue Ice or 4ºC

-20ºC

Detects ~90kDa. Does not cross-react with GIT2.

1. Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475. 2. Manabe R., et al. (2002) J. Cell Sci. 115: 1497-1510. 3. Zhang H., et al. (2003) J. Cell Biol. 161: 131-142. 4. Goehler H., et al. (2004) Mol. Cell 15: 853-865. 5. Mende-Mueller L.M., et al. (2001) J. Neurosci. 21: 1830-1837.

Human | Mouse | Rat

Cytoplasm

IgG1