Copper Transporting ATPase 2 Antibody

Copper Transporting ATPase 2 Antibody

• Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver, characterized by neurological symptoms and hepatic damage.
• Description: Mouse Anti-Human Copper Transporting ATPase 2 Monoclonal IgG1
• Specifications: Detects ~160kDa in rat brain membrane preparations.
• Alternative Name: ATP7B Antibody, ATPase Cu++ transporting beta polypeptide Antibody, ATPase Cu(2+) transporting beta polypeptide Antibody, Copper pump 2 Antibody, Copper transporting ATPase 2 Antibody, PWD Antibody, Toxic milk Antibody, tx Antibody, WC1 Antibody, WD Antibody, Wilson disease associated protein Antibody, WND Antibody, WND/140 kDa Antibody
• Synonyms: Copper Transporting ATPase 2 Antibody, Clone L62/29
• UNSPSC: 12352203
• Gene ID: 540
• Swiss Prot: B7ZLR4
• Accession Number: NP_000044.2
• Cellular Locus: Cytoplasm | Mitochondrion | Golgi Apparatus | Trans-Golgi Network Membrane
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2
• Target: ATP7B
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: L62/29 (Formerly sold as S62-29)
• Conjugation: Unconjugated
• Validated Applications: WB, IHC, ICC/IF, IP
• Purification: Protein G Purified
• Limit Of Detection: 1 µg/ml of SMC-399 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Weight: 0.012
• Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Tanzi R.E., et al. (1993) Nature Genetics. 5: 344-350. 2. Ghr/nlm.gov/gene/ATP7B