N-terminal Arginylation Antibody, Clone 11G1

• Background:

  N-terminal arginylation is a conserved post-translational modification that adds arginine residues to proteins, primarily via the action of arginyltransferase ATE1. This modification plays a pivotal role in embryonic development, cytoskeletal regulation, and cellular stress responses. In the context of neurodegeneration, N-terminal arginylation is essential for maintaining neuronal proteostasis and responding to endoplasmic reticulum and oxidative stress. It facilitates the degradation of misfolded proteins and modulates the actin cytoskeleton, both of which are critical for synaptic function and neuronal survival. Disruption of arginylation pathways has been linked to neurodevelopmental disorders and may contribute to the progression of diseases such as ALS and Alzheimer’s. As such, N-terminal arginylation represents a promising avenue for therapeutic exploration in neuroscience.

• Description:

  Mouse Anti-N-terminal Arginylation Monoclonal IgG3

• Specifications:

  Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.

• Product Name Alternative:

  N-terminal Arginine, N-terminal Arginylation, N-terminal Arginylated, N terminal Arginine, N terminal Arginylation, N terminal Arginylated, Amino-terminal Arginine, Amino-terminal Arginylation, Amino-terminal Arginylated, Amino terminal Arginine, Amino terminal Arginylation, Amino terminal Arginylated

• Synonyms:

  N-terminal Arginylation Antibody, Clone 11G1

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Cellular Locus:

  Endoplasmic Reticulum

• Host:

  Mouse

• Species Reactivity:

  Species Independent

• Immunogen:

  Synthetic N-terminal arginylated peptide conjugated to KLH

• Target:

  N-terminal Arginylation

• Clonality:

  Monoclonal

• Isotype:

  IgG3

• Clone:

  11G1

• Conjugation:

  Unconjugated

• Type:

  Monoclonal

• Applications:

  WB

• Validated Applications:

  WB

• Field of Research:

  Cell Signaling | Post-translational Modifications | Arginylation

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-265 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.

• Background Reference 01:

  1. Saha S. and Kashina A. (2011) Dev Biol. 385 (1) : 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

• Species:

  Species Independent

• CAS Number:

  9007-83-4

• Location:

  Endoplasmic Reticulum

• Isotype:

  IgG3