N-terminal Arginylation Antibody, Clone 4A9: APC

• Background:

  N-terminal arginylation is a post-translational modification catalyzed by arginyltransferase ATE1, in which an arginine residue is added to the N-terminus of proteins. This process plays a critical role in protein quality control, cellular stress responses, and neurodevelopment. In the nervous system, N-terminal arginylation regulates the degradation of misfolded or damaged proteins via the N-degron pathway, thereby maintaining proteostasis—a function essential for neuronal survival. Recent studies have linked impaired arginylation to neurodegenerative diseases such as Alzheimer’s and Parkinson’s, where accumulation of misfolded proteins and disrupted stress responses are key pathological features. Arginylation also modulates cytoskeletal dynamics and synaptic plasticity, suggesting broader roles in neuronal connectivity and cognitive function. Dysregulation of ATE1 activity has been associated with increased vulnerability to oxidative stress and endoplasmic reticulum stress, both of which are implicated in neurodegeneration. Given its multifaceted role in neuronal health, N-terminal arginylation represents a promising target for therapeutic intervention and biomarker development in neurodegenerative disease research.

• Description:

  Mouse Anti-N-terminal Arginylation Monoclonal IgG1

• Specifications:

  Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

• Product Name Alternative:

  N-terminal Arginine, N-terminal Arginylation, N-terminal Arginylated, N terminal Arginine, N terminal Arginylation, N terminal Arginylated, Amino-terminal Arginine, Amino-terminal Arginylation, Amino-terminal Arginylated, Amino terminal Arginine, Amino terminal Arginylation, Amino terminal Arginylated

• Synonyms:

  N-terminal Arginylation Antibody, Clone 4A9: APC

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Cellular Locus:

  Endoplasmic Reticulum

• Host:

  Mouse

• Species Reactivity:

  Species Independent

• Immunogen:

  Synthetic N-terminal arginylated peptide conjugated to KLH

• Target:

  N-terminal Arginylation

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  4A9

• Conjugation:

  APC

• Type:

  Recombinant Monoclonal

• Applications:

  WB | FCM | FACS

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Cell Signaling | Post-translational Modifications | Arginylation

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-263 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000) ; FCM (1:50) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  95.46mM Phosphate, 2.48mM MES and 2mM EDTA

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

• Background Reference 01:

  1. Saha S. and Kashina A. (2011) Dev Biol. 385 (1) : 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

• Species:

  Species Independent

• CAS Number:

  9007-83-4

• Location:

  Endoplasmic Reticulum

• Isotype:

  IgG1