HIF1 alpha Antibody

HIF1 alpha Antibody

• Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
• Description: Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1
• Specifications: Detects ~116kDa. Specific for HIF1Alpha.
• Product Name Alternative: ARNT interacting protein Antibody, HIF1A Antibody, Hypoxia inducible factor 1 alpha Antibody, MOP1 Antibody, PASD8 Antibody
• Synonyms: HIF 1 alpha Antibody, Clone ESEE122
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 15251
• Swiss Prot: Q61221
• Accession Number: NP_034561.2
• Cellular Locus: Cytoplasm | Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat, Bovine
• Immunogen: Mouse recombinant fragment corresponding to amino acids 329-531
• Target: HIF 1 alpha
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: ESEE122
• Conjugation: Unconjugated
• Validated Applications: WB, IHC, ICC/IF, ELISA
• Purification: Protein G Purified
• Limit Of Detection: 1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4. 3. Jaakkola P., et al. (2001) Science 292: 468-72. 4. Maxwell P.H., et al. (1999) Nature 399: 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9. 7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.