HSP90 Antibody, Clone 4F3.E8

HSP90 is a highly conserved and abundantly expressed molecular chaperone that plays a central role in maintaining protein homeostasis, particularly in the nervous system. Present in all eukaryotic cells, HSP90 exists in two major cytosolic isoforms—HSP90α and HSP90β—which share 85% sequence identity but differ in oligomeric state and regulatory function. Despite its classification as a heat shock protein, HSP90 is constitutively expressed at high levels, comprising up to 2% of total cytosolic protein in unstressed cells. It is essential for the folding, maturation, and stabilization of a wide range of client proteins, many of which are involved in neuronal signaling, synaptic plasticity, and stress response. These include kinases (e.g., c-Raf), transcription factors (e.g., p53), and steroid hormone receptors. In neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s, HSP90 is implicated in both protective and pathological processes. It stabilizes misfolded proteins and prevents aggregation, but can also shield aberrant proteins from degradation, contributing to disease progression. HSP90’s interaction with co-chaperones like Cdc37 and p23 forms complexes that regulate the fate of client proteins, making it a key node in proteostasis networks. Pharmacological inhibition of HSP90—using compounds like geldanamycin—has shown promise in modulating protein quality control pathways and reducing toxic protein accumulation in neurodegenerative models.

Mouse Anti-Human HSP90 (total) Monoclonal IgG2a

9007-83-4

Detects ~90kDa. This antibody detects both α and β forms of HSP90 equally well.

HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

HSP90 Antibody, Clone 4F3.E8

12352203

Non-hazardous

Non-hazardous

3320

P07900

NP_001017963.2

Cytoplasm | Melanosome

Recombinant Human HSP90 purified from E.coli

HSP90

4F3.E8

Unconjugated

Monoclonal

WB | IHC | ICC/IF | IP | ELISA

Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers

Protein G Purified

0.5 µg/ml of SMC-149 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

1 mg/ml

WB (1:2000), IHC (1:100), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

0.012

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Nemoto T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Nemoto, T. (1997) Biochem and Mol. Bio Intl. 42 (5): 881-889.

Blue Ice or 4ºC

-20ºC

Detects ~90kDa. This antibody detects both α and β forms of HSP90 equally well.

1. Nemoto T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Nemoto, T. (1997) Biochem and Mol. Bio Intl. 42 (5) : 881-889.

Human | Mouse | Rat | Plant | Wheat (Triticum spp.)

Cytoplasm | Melanosome

IgG2a