HSP70/HSC70 Antibody, Clone BB70: HRP

• Background:

  HSP70 proteins are a highly conserved family of 70-kDa molecular chaperones essential for maintaining protein homeostasis across all domains of life. In eukaryotes, HSP70 genes form a multigene family, with isoforms localized to the cytosol, nucleus, mitochondria, endoplasmic reticulum, and chloroplasts. These chaperones are either constitutively expressed (HSC70) or stress-inducible (HSP70), and share over 50% sequence identity across species. Functionally, HSP70s bind ATP with high affinity and exhibit ATPase activity that is stimulated by interaction with unfolded or misfolded proteins. The N-terminal domain mediates ATP binding, while the C-terminal domain is responsible for substrate recognition. This ATP-driven cycle enables HSP70s to bind and release hydrophobic regions of nascent or damaged proteins, preventing aggregation and facilitating proper folding, transport, and assembly. In neuroscience, HSP70 and HSC70 are critical for neuronal survival under proteotoxic stress—conditions commonly associated with neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s disease. These chaperones stabilize misfolded proteins, support autophagic clearance, and modulate apoptotic signaling pathways. Their ability to interact with co-chaperones and degradation machinery further enhances their role in maintaining neuronal proteostasis. Given their central role in stress response and protein quality control, HSP70/HSC70 are promising therapeutic targets and biomarkers in neurodegeneration research.

• Description:

  Mouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a

• Specifications:

  Detects ~72 (HSP) and ~73kDa (HSC).

• Product Name Alternative:

  HSPA1A, HSPA1B, HSPA1, HSPA8, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSC70, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein

• Synonyms:

  HSP70/HSC70 Antibody, Clone BB70: HRP

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  423504

• Swiss Prot:

  P08106

• Accession Number:

  NP_001006686.1

• Cellular Locus:

  Cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig, Pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

• Immunogen:

  Chicken HSP70/HSP90 complex

• Target:

  HSP70/HSC70

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  BB70

• Conjugation:

  HRP

• Type:

  Monoclonal

• Applications:

  WB | ICC/IF | IHC | IP

• Validated Applications:

  WB, IHC, ELISA

• Field of Research:

  Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers | Cancer | Heat Shock

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-106 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:200), ICC/IF (1:200) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.2

• Buffer:

  73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Zho J. (1998) Cell 94 : 471-480. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11):6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408(6808): 101-106.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~72 (HSP) and ~73kDa (HSC) .

• Background Reference 01:

  1. Zho J. (1998) Cell 94 : 471-480. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1) : 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13 (2) : 869-876. 8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16 (11) :6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408 (6808) : 101-106.

• Species:

  Human | Mouse | Rat | Bovine | Sheep | Dog | Beluga | Fish | Guinea Pig (Cavia porcellus) | Pig | Hamster | Rabbit | Chicken | African clawed frog (Xenopus laevis) | Fruit Fly (Drosophila melanogaster) | Yeast

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Isotype:

  IgG2a

• Immunogen Species:

  Chicken