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Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit

CAT: 0749-KTE70163-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTE70163-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels.MAC30 expression in all normal human tissues examined, with highest expression in testis and stomach and lowest expression in thyroid, thymus, and adipose tissue.In situ hybridization detected MAC30 in normal pancreatic acini, islets, and large ducts. Immunohistochemical analysis showed MAC30 protein in the cytoplasm of peripheral pancreatic islet cells, in endothelial and smooth muscle cells of blood vessels, and in nerves, monocytes, and fibroblasts. In esophagus, MAC30 was detected in cytoplasm of mucosal cells, particularly in the superficial and intermediate epithelial layers. Gastric submucosa and muscularis and normal colonic epithelium exhibited staining patterns similar to that of esophagus.
Description
This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30.
CAS Number
7732-18-5
Product Name Alternative
TMEM97/MAC30; MAC30
Gene ID
69071
Accession Number
Q8VD00
Reactivity
Mouse
Tag
TMEM97/MAC30
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM97/MAC30. No significant cross-reactivity or interference between Mouse TMEM97/MAC30 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30 in samples. An antibody specific for TMEM97/MAC30 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM97/MAC30 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM97/MAC30 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM97/MAC30 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
TMEM97/MAC30; MAC30
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/Q8VD00
Calibration Range
Please inquire

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Alternative Products

CatalogName
302798TMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) discontinued
302798-HRPTMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (HRP) discontinued
302798-APTMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (AP) discontinued
302798-BiotinTMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (Biotin) discontinued
302798-APCTMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (APC) discontinued
302798-PETMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (PE) discontinued
302798-FITCTMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (FITC) discontinued
302798-ML550TMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (MaxLight 550) discontinued
302798-ML650TMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (MaxLight 650) discontinued
302798-ML405TMEM97 (Transmembrane Protein 97, Protein MAC30, TMEM97, MAC30) (MaxLight 405) discontinued

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